Structural basis of NPR1 in activating plant immunity – Nature

  • Delaney, T. P., Friedrich, L. & Ryals, J. A. Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance. Proc. Natl Acad. Sci. USA 92, 6602–6606 (1995).

    ADS 
    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Shah, J., Tsui, F. & Klessig, D. F. Characterization of a salicylic acid-insensitive mutant (sai1) of Arabidopsis thaliana, identified in a selective screen utilizing the SA-inducible expression of the tms2 gene. Mol. Plant Microbe Interact. 10, 69–78 (1997).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Cao, H., Bowling, S. A., Gordon, A. S. & Dong, X. Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance. Plant Cell 6, 1583–1592 (1994).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Glazebrook, J., Rogers, E. E. & Ausubel, F. M. Isolation of Arabidopsis mutants with enhanced disease susceptibility by direct screening. Genetics 143, 973–982 (1996).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Ryals, J. et al. The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor I kappa B. Plant Cell 9, 425–439 (1997).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Cao, H., Glazebrook, J., Clarke, J. D., Volko, S. & Dong, X. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell 88, 57–63 (1997).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Backer, R., Naidoo, S. & van den Berg, N. The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) and related family: mechanistic insights in plant disease resistance. Front. Plant Sci. 10, 102 (2019).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Silva, K. J. P., Mahna, N., Mou, Z. & Folta, K. M. NPR1 as a transgenic crop protection strategy in horticultural species. Hortic. Res. 5, 15 (2018).

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • Stogios, P. J., Downs, G. S., Jauhal, J. J., Nandra, S. K. & Prive, G. G. Sequence and structural analysis of BTB domain proteins. Genome Biol. 6, R82 (2005).

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • Stogios, P. J. & Prive, G. G. The BACK domain in BTB-kelch proteins. Trends Biochem. Sci. 29, 634–637 (2004).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Canning, P. et al. Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases. J. Biol. Chem. 288, 7803–7814 (2013).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Zhuang, M. et al. Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases. Mol. Cell 36, 39–50 (2009).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Errington, W. J. et al. Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase. Structure 20, 1141–1153 (2012).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Gorina, S. & Pavletich, N. P. Structure of the p53 tumor suppressor bound to the ankyrin and SH3 domains of 53BP2. Science 274, 1001–1005 (1996).

    ADS 
    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Li, J., Mahajan, A. & Tsai, M. D. Ankyrin repeat: a unique motif mediating protein-protein interactions. Biochemistry 45, 15168–15178 (2006).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Sedgwick, S. G. & Smerdon, S. J. The ankyrin repeat: a diversity of interactions on a common structural framework. Trends Biochem. Sci. 24, 311–316 (1999).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Wang, W. et al. Structural basis of salicylic acid perception by Arabidopsis NPR proteins. Nature 586, 311–316 (2020).

    ADS 
    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Mou, Z., Fan, W. & Dong, X. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes. Cell 113, 935–944 (2003).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Canet, J. V., Dobon, A., Roig, A. & Tornero, P. Structure-function analysis of npr1 alleles in Arabidopsis reveals a role for its paralogs in the perception of salicylic acid. Plant Cell Environ. 33, 1911–1922 (2010).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Bombarda, E., Cherradi, H., Morellet, N., Roques, B. P. & Mely, Y. Zn2+ binding properties of single-point mutants of the C-terminal zinc finger of the HIV-1 nucleocapsid protein: evidence of a critical role of cysteine 49 in Zn2+ dissociation. Biochemistry 41, 4312–4320 (2002).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Hol, W. G. Effects of the alpha-helix dipole upon the functioning and structure of proteins and peptides. Adv. Biophys. 19, 133–165 (1985).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Tada, Y. et al. Plant immunity requires conformational changes of NPR1 via S-nitrosylation and thioredoxins. Science 321, 952–956 (2008).

    ADS 
    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Manohar, M. et al. Identification of multiple salicylic acid-binding proteins using two high throughput screens. Front. Plant Sci. 5, 777 (2014).

    PubMed 

    Google Scholar 

  • Ding, Y. et al. Opposite roles of salicylic acid receptors NPR1 and NPR3/NPR4 in transcriptional regulation of plant immunity. Cell 173, 1454–1467 (2018).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Waterhouse, A. et al. SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46, W296–W303 (2018).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Saleh, A. et al. Posttranslational modifications of the master transcriptional regulator NPR1 enable dynamic but tight control of plant immune responses. Cell Host Microbe 18, 169–182 (2015).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Maier, F. et al. NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 (NPR1) and some NPR1-related proteins are sensitive to salicylic acid. Mol. Plant Pathol. 12, 73–91 (2011).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Hermann, M. et al. The Arabidopsis NIMIN proteins affect NPR1 differentially. Front. Plant Sci. 4, 88 (2013).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Lebel, E. et al. Functional analysis of regulatory sequences controlling PR-1 gene expression in Arabidopsis. Plant J. 16, 223–233 (1998).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Kachroo, A. & Kachroo, P. Fatty acid-derived signals in plant defense. Annu. Rev. Phytopathol. 47, 153–176 (2009).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Fu, Z. Q. & Dong, X. Systemic acquired resistance: turning local infection into global defense. Annu. Rev. Plant Biol. 64, 839–863 (2013).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Wang, D., Amornsiripanitch, N. & Dong, X. A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS Pathog. 2, e123 (2006).

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • Pape, S., Thurow, C. & Gatz, C. The Arabidopsis PR-1 promoter contains multiple integration sites for the coactivator NPR1 and the repressor SNI1. Plant Physiol. 154, 1805–1818 (2010).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Sievers, F. et al. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol. Syst. Biol. 7, 539 (2011).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Mastronarde, D. N. Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36–51 (2005).

    PubMed 
    Article 

    Google Scholar 

  • Scheres, S. H. RELION: implementation of a Bayesian approach to cryo-EM structure determination. J. Struct. Biol. 180, 519–530 (2012).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Rohou, A. & Grigorieff, N. CTFFIND4: fast and accurate defocus estimation from electron micrographs. J. Struct. Biol. 192, 216–221 (2015).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat. Methods 14, 290–296 (2017).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D 60, 2126–2132 (2004).

    PubMed 
    Article 
    CAS 

    Google Scholar 

  • Afonine, P. V. et al. Real-space refinement in PHENIX for cryo-EM and crystallography. Acta Crystallogr. D 74, 531–544 (2018).

    CAS 
    Article 

    Google Scholar 

  • Bepler, T. et al. Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs. Nat. Methods 16, 1153–1160 (2019).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Kabsch, W. XDS. Acta Crystallogr. D 66, 125–132 (2010).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Bligh, E. G. & Dyer, W. J. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917 (1959).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Li, C., Tan, B. K., Zhao, J. & Guan, Z. In vivo and in vitro synthesis of phosphatidylglycerol by an Escherichia coli cardiolipin synthase. J. Biol. Chem. 291, 25144–25153 (2016).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Spoel, S. H. et al. Proteasome-mediated turnover of the transcription coactivator NPR1 plays dual roles in regulating plant immunity. Cell 137, 860–872 (2009).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Zavaliev, R., Mohan, R., Chen, T. & Dong, X. Formation of NPR1 condensates promotes cell survival during the plant immune response. Cell 182, 1093–1108 (2020).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Clough, S. J. & Bent, A. F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743 (1998).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676–682 (2012).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Zavaliev, R. & Epel, B. L. Imaging callose at plasmodesmata using aniline blue: quantitative confocal microscopy. Methods Mol. Biol. 1217, 105–119 (2015).

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • #Structural #basis #NPR1 #activating #plant #immunity #Nature

    Leave a Comment

    Your email address will not be published.